巴马小型猪群体遗传结构的随机扩增多态DNA分析
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Q953

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国家“九五”重点科技攻关项目!(96A-23-06-01),国家自然科学基金!(NO 39830190),面上项目!(39770


Analysis of Genetic Constrution of Population in Bama Miniature Pigs by RAPD
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    摘要:

    目的分析巴马小型猪的群体遗传结构。方法应用经过筛选的 31条引物对巴马小型猪个体基因组DNA进行RAPD扩增,利用从internet网上下载的软件RAPD istance Package ver-sion1.04软件对实验结果进行分析,计算不同个体间的相似系数。结果经RAPD反应,共扩增出275条带,其中多态性带85条,多态性带频率在0~66.7%之间,平均为27.7%。不同个体猪拥有的RAPD条带具有差异,但拥有相同条带个体猪比率较高。该群体猪的相似系数(F)为0.928(0.78~0.97),平均等位基因频率(q)为0.732,最低平均杂合率(H)为0.286。结论巴马小型猪个体具有较好的遗传一致性和遗传稳定性。

    Abstract:

    Objective To analyse the genetic construction of population in Bama miniature pigs. Methods 31 selected single primers were used to amplify genomic DNA of Bama miniature pigs. The amplified fragments were analysed by the RAPD istance package version 1.04 and the similarity index was calculated. Results 275 bands were obtained in which polymorphisic bands were 85, the frenquency of polymorphisic bands were between 0-66.7%. Different individual pig has various bands, but the frenquency of common band was higher. Based on RAPD patters similarity index (F) calculated was 0.928 (0.78-0.96), mean allelic frequency (q) was 0. 732, and minimun heterozygosites (H) was 0.268. Conclusion Bama,miniature pigs reached a higher degree of homogeneity than ordinary bred pigs.

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吴丰春,魏泓,王爱德.巴马小型猪群体遗传结构的随机扩增多态DNA分析[J].中国实验动物学报,2000,(4):235~240.

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