Objective To screen the available MHV combinations for preparing detective kit. Methods Five MHVs were used alone and in different combinations for studying the reactions with standard MHV lines by means of ELISA. The specificity and sensitivity were compared. Results 238 semples of conventional mice from different units were detected. The rate of infection was very high (59%-87%). Conclusion MHV-HU79 isolated in Shanghai of China can be used in the diagnostic kit for MHV antibody.