Objective To observe the injury and permeability of intestine, together with the changes of peroxidation and TNF and IL 6 concentrations after hemorrhagic shock in rats. Methods At 0, 2, 6, 12, 24 and 48 hours after being subjected to sham shock, or 70 minutes of 30mmHg shock, rats were sacrificed. The SOD activity and the MDA, TNF and IL 6 concentrations in serum and intestine were detected, the histology and bacteria translocation of intestine were also observed. Results The serum MDA concentration was increased at 0-2h after shock and SOD activity was increased almost all the detected time. In the intestine, the MDA concentration increased at 0-24h, and SOD activity decreased at 0-12h. The serum TNF concentration was increased at 6-48h after shock while increased at 0-12h in intestine. No change was observed of IL 6 concentration in both serum and intestine. Extensive sheding of mucosa epithelia were observed at 2h after shock and the bacteria were observed at 6-12h in lamina propria. The indigenous bacteria of intestine were detected at mesenteric lymph nodes and other visceral organs at 6-48h after shock. Conclusion The SOD activity decrease was one of the reasons in intestine injury after hemorrhagic shock. The early phase increase of serum TNF came from the injured intestine.