Objective In order to study the effect of the genotype of different strains on mouse embryo cryopreservation in the glycerol vitrification solution.Methods\ The 6 5mol/L glycerol and 6% (w/v) BSA were used as the vitrification solution for the cryopreservation of morulae and blastocysts embryos of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse.Results and Conclusion\ The rates of in vitro development of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse embryos to expanded blastocysts ranged from 31 3% to 88% after vitrification.The rates of CBA,NOD,C 57 BL/6J,ICR and CD1 mouse embryos to normal fetuses was 21%,23 5%,11%,38%,35 5%.The rates of in vitro development of CBA,NOD,C 57 BL/6J6,ICR and CD1 mouse morulae and early blastocysts were significantly higher than expanded blastocystst.The in vitro development and in vivo development were significantly influenced by inbred strain,closed colony and different stages of mouse embryo.The result of mouse embryo cryopreservation in the vitrification solution may be connected with the different genotype of the different strains and different stages of mouse embryo.