Objective To study the sterilization effect of glutaraldehyde compound disinfectant on some swine viruses, and to determine the optimum disinfectant concentration.Methods\ PPV,PRRSV and PRV,treated with glutaraldehyde compound disinfectant at different concentration,were inoculated on cell monolayers.The optimum disinfectant concentration was determined according to the cytopathologic effects.HCV reacted with the disinfectant at different concentration was inoculated into rabbits through vein injection,the optimum concentration was decided according to the body temperature changes.Results\ PPV, PRRSV and PRV could be disinfected effectively by 1% disinfectant in 20 min.And HCV could be killed effectively by 0\^5% disinfectant in 10 min.Conclusion\ Glutaraldehyde compound disinfectant possesses good sterilization effect with the concentration of 1% and 0\^5% to some swine viruses,and is suitable for disinfecting the stock farm,hospital and epidemic area of infectious diseases.It is a kind of practicable method of in vitro experiment to detect and judge the sterilization effect of disinfectant with the cell infection method.