R587.2
云南省自然科学基金项目 (1999C0 0 97M)
目的 建立糖尿病性视网膜并发症 (DiabeticRetinopathy ,DR)动物模型。方法 本研究选用 4只健康成熟的雄性恒河猴分别以剂量为 6 0mg kg、4 5mg kg静脉一次注射及 30mg kg静脉二次注射 (中间间隔 15d)链脲佐菌素 (Streptozotocin ,STZ)。结果 使用不同剂量的STZ注射恒河猴 ,分别造成了胰岛素依赖性糖尿病 (InsulinDepen dentDiabetesMellitus ,IDDM)及慢性持续性高血糖症 (StateofChronicHyperglycemia,SCH)两种类型的模型 ,从而诱导出不同程度的DR。眼底荧光造影结果显示∶用药后 6 3～ 91d4只实验猴均出现不同程度的视网膜病 ,分别显示早期眼底微血管动脉扩张 ,视网膜出血瘤 ,微血管瘤及新生血管、晚期白内障等。结论 本研究所建立的糖尿病性视网膜并发症模型 ,对于进一步研究糖尿病及其并发症的发病机理 ,筛选及开发治疗糖尿病性视网膜病的新药及药物安全性评价将会具有广阔的应用前景
Objective To establish the model of diabetic retinopathy complication in rhesus monkey. Methods Four adult, healthy and male rhesus monkeys were injected intravenously with different STZ dosage(one of the four with 60 mg/kg, one with 45 mg/kg, the other two with 30 mg/kg twice fifteen days apart). Result All of the monkeys were made animal models of the insulin dependent diabetes mellitus(IDDM) and the state of chronic hyperglycemia(SCH). These diabetic monkeys had long term higher preprandial plasma glucose, and they showed tiny blood vessel dilation in eyeground, early retinal hemorrhage dot, tiny angioma and cataract, during 63-91days after inducing STZ. Conclusion Animal model of diabetic retinopathy complication disease was established. It would be useful for studying the mechanism of diabetes mellitus and its complication, and observing the efficiency, safety of the diabetic drugs instead of human being.
唐东红,代解杰,蒋禄芝,孙晓梅,匡德宣,高家红,江勤芳,张学宁. STZ诱发实验恒河猴糖尿病性视网膜并发症模型的建立[J].中国实验动物学报,2002,(3):173~176.
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