The technique route for making transgenic animals by somatic cell mediated transgene has been developed based on the technique of nuclear transfer. Although it came into being for only about 4-5years, it has shown broad application prospect and great potential. In this review, we compared advantages and disadvantages of different methods for making transgenic animals, expounded the basic technique route for making transgenic animals by somatic cell mediated transgene, emphasized the key points of somatic cell transfection and cloning of integrated cells, and the limitation and potential application of this technique.