Objective The effects of glucose, pyruvate and lactate on in vitro development of bovine embryos produced in vitro in chemical defined culture system (SOF+PVA) were observed in this research. Method Bovine oocytes were matured and fertilized in vitro, then the fertilized ova were cultured in vitro in chemical defined culture medium. Results Experiment 1: Bovine IVF embryos were cultured in SOF+PVA that was not supplemented with glucose, then were cultured in SOF+PVA supplemented with 0, 1.50, 3.30 and 5.00mmol/L glucose respectively after being cultured 120 h, the embryos in control group were cultured in SOF+PVA supplemented with 1.50 mmol/L glucose during all the culture time. The results showed that the development rates of blastocysts were 9.2% a, 12.1%, 19.2% b, 18.9% and 11.7% respectively (a