Objective To breed and establish a new wild derived inbred strain of mice trapped in China, and select its important biological characteristics for enriching present gene bank of laboratory animals. Methods Wild mice were inbred strictly according to the international standards, that is, brother sister cross breeding by the same litters. Results Through 12 years of inbreeding, TW (Tianjin Wild) mice were inbred to F22 generation. And mouse strains from other 15 trapping localities were all interrupted or stopped by genetic reasons. Conclusion This study may enrich international gene bank of laboratory mouse and lay a foundation for establishing the laboratory mouse model of anti-lung tumors.