Objective Thirty three indexes of hematological and biochemical parameters in eighty mongolian gerbils were mensurated and compared between different sexes and regions. Methods The blood of laboratory animals was taken from eyeball after fasting of 12 hours for measuring hematological and biochemical parameters. Results Most hematological and biochemical parameters were influenced by age difference,and there were no significant differences by sex except GLU,CK,CRE,UA,TG,GOT,CHO,ALP,LDH,RBC,HGB,MCHC,PLT and WBC. Most hematological and biochemical parameters were also different by region difference. Conclusion The rearing condition and age difference were important influencing factors of these indexes.