Objective To describe the pathology of liver tumors in rabbits induced by diethylnitrosamine and the expression of c myc, p53 genes and AFP in the tumors and pericarcinomatous tissues. Method 25 nodules in 5 rabbit livers and 5 pieces of pericarcinomatous tissues each from one liver were observed by light microscopy and electron microscopy, and the expressions of c myc gene, p53 gene and AFP were also observed in these tissues with immunohistochemical staining.Results and Conclusion There were 10 nodules of adenomatous hyperplasia, 11 of well differentiated hepatocellular carcinoma(HCC)and 4 of moderate differentiated HCC among the 25 nodules. The tumorigenesis of HCC in rabbits induced by diethylnitrosamine was related to the over expressions of c myc gene, AFP and the mutation of p53 gene. C myc over expression appeared first and p53 mutation the latest during the tumorigenesis of HCC. AFP over expression reached the top positive reaction ratio(93 3%) among the 3 detected parameters.