[Objective] To research cold or hot constitution of four species of mouse.[Methods] eight to nine-week-old kunming mice BALB/c mice , C57BL/6Jmice , ICR mice and four to five-week-old kunming mice were inspected their biological characteristics with a simultaneous systematic inspection.The four species of mice are assessed by a same criterion about their constitution whether it is prone to cold or hot. The satime BALB/c mice treated with pill of Shenguilizhong and reserpine were used negtive control. [Results] 1. Four to five-week old kunming mice is compared with eight to nine-week-old kunming mice.The former constitution is obviously prone to hot; 2. Comparsion between BALB/c mice and C57BL/6J mice,the former is prone to cold; 3. Eight to nine-week-old male BALB/c mice and C57BL/6J mice (include male and female) are compared with eight to nine-week-old corresponding sex kunming mice respectively.Each group do not have obivious difference. Eight to nine-week-old female BALB/c mice is compared with eight to nine-week-old female kunming mice. The former is prone to cold. 4. Eight to nine-week-old ICR mice is prone to hotter than eight to nine-week-old BALB/c mice and C57BL/6J mice; The eight to nine-week-old male ICR mice is prone to hotter than the eight to nine-week-old male kunming mice.[Conclusion] The four species of mice have the difference of cold or hot constitution.