目的建立兔VX2肿瘤脑内种植动物模型,观察其生长特性。方法采用兔脑内VX2肿瘤组织块种植法将VX2肿瘤组织块种植入24只成年New Zealand大白兔右侧大脑皮质内,用B超检测肿瘤的生长情况,在实验兔在肿瘤种植后第13、171、9、21、232、5天取材,进行组织学观察。并观察实验兔在种植VX2肿瘤后的生存期及出现厌食、偏瘫等神经系统体征和死亡的时间。结果VX2肿瘤组织块种植入脑内后荷瘤兔的中位生存期为24.5 d,平均生存期为24.8 d,肿瘤体积随种植后的时间在对数坐标中接近一条直线。VX2肿瘤种植入兔脑17 d后血供较丰富、呈鱼肉状生长,与正常脑组织边缘界限不清楚,第17~19天肿瘤中心出现坏死并出现腹腔内转移。光镜下从VX2肿瘤种植后第17天开始肿瘤细胞向正常脑组织浸润,并形成瘤巢,瘤周脑组织形成水肿带。结论在缺乏兔源性脑肿瘤的情况下,采用兔VX2肿瘤组织块颅内种植能较好模拟颅内肿瘤生长,为对脑肿瘤的某些实验研究提供条件。
Objective To establish a model of brain tumor by VX2 carcinoma implantation and observe the growth characteristics of the tumor.Methods VX2 tumor mass was implanted into the right parietal lobe brain 24 New Zealand rabbits.B-mode ultrasonography was proceeded to observe the growth and the volume of tumors.Animals were sacrificed at day 13, 17,19,21,23 and 25 after tumor implantation,respectively.Sections were cut for histopathological examination.The survival time and significant neurological alterations such as difficult feeding and hemiplegia were recorded.Results The median survival time of rabbits with implanted VX2 tumor was 24.5 days and the average was 24.8 days.The log-transformed plots of tumor volume approximate a straight line.17 days after tumor implantation,the VX2 tumors showed a brown coloration,appeared like fish and metastasized to the abdomen.The tumor cells infiltrated into the surrounding brain tissues and peri-tumor edema appeared since day 17 after tumor implantation.Conclusion Implantation of VX2 tumor into the rabbit brain is an useful model for human brain tumor research.
李飞,冯华,林江凯,孟辉,吴南.兔脑种植VX2肿瘤动物模型的建立[J].中国实验动物学报,2006,(3):193~196.
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