目的延长糖尿病模型大鼠生存期,动态观察糖尿病视网膜病变(DR)的形成和发展过程。方法雄性SD大鼠70只,随机分成对照组(20只)和模型组(50只),采用链脲佐菌素(STZ)60 mg/(kg.bw)体重腹腔1次注射造模,分别于69、、12月时处死取眼球,采用视网膜微血管消化铺片技术观察糖尿病视网膜病变的微血管形态学改变。结果糖尿病大鼠DR样病变随着病程的延长病变呈多样性改变,以12月DR出现的小动脉硬化尤为严重。结论糖尿病大鼠生存期的延长对糖尿病视网膜病变的研究有着积极的意义。
Objective To extend the survival time of diabetic rat in order to observe the formation and development of diabetic retinopathy(DR).Method 70 SD rats were divided randomly into control group(n = 20) and DR group(n = 50).Streptozotocin(STZ) in a dose of 60 mg/kg was injected into peritoneal cavity to induce diabetes mellitus.DR group rats were killed at 6~(th),9~(th) and 12~(th) month,respectively.Retina microvasculature was digested,stretched,PAS stained and used to examine the DR microvascular alteration.Result Diversified lesions occurred with the progressing of survival time.The most serious arteriolosclerosis occurred at 12~(th) month.Conclusion The prolongation of survival time of diabetic rats is beneficial for investigation of the progression of diabetic retinopathy.
李鸿,潘琳.延长糖尿病模型大鼠生存期对糖尿病视网膜病变的影响[J].中国实验动物学报,2006,(3):201~203.
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