Objective The aim of this study was to determine the blood physiological and biochemical parameters of Tibet mini-pigs raised in subtropical environment.Methods Nine physiological and 9 biochemical blood parameters in original and F1 generations of Tibet mini-pigs raised in subtropical area were measured and the differences between generations and between both sexes were analyzed.Results Among the biochemical indexes,there was a significant difference in AST between male and female pigs of original generation,and TG,ALB,BUN and CHOL between male and female pigs in the first generation.In the blood physiological indexes,there was no significant difference between male and female pigs of original generation,but there was significant difference in MONO between male and female pigs of the first generation.Conclusion Tibet mini-pigs are adapted to the subtropical environment through local domestication over the past two years.Compared with other animals and human beings,Tibet mini-pigs as laboratory animals are more closely resembling human beings than dogs and monkeys in regards of some physiological and biochemical parameters,and more suitable to be used in experimental studies instead of other animals such as dogs and monkeys.