Objective To establish a rat model of chronic renal failure in two ways and observe the leptin expres-sion in the renal tissue of the rats.Methods 1.Chronic renal failure was induced by 5 /6 nephrectomy(Platt Method).2.Rats were fed with 2.5% adenine to cause chronic renal failure.The changes of blood BUN,Ca2 +,P5 + and Scr were as-sayed.HE and Masson staining were used for pathological examination,and immunofluorescence microscopy was used to ob-serve the leptin expression in the renal tissue of the model ...