ObjectiveTo clone the full length cDNA of Uncv gene in mice and to express the gene in eukaryotic cells.MethodsRT-PCR assay was applied to clone the full length coding region of the Uncv gene and constructed its expression plasmid pcDNA 3.1-Flag/Uncv. The recombinant plasmid was transfected into HeLa cells and the fusion protein was identified by Western blot analysis.ResultsThe complete coding sequence was obtained and cloned into the pcDNA 3.1-Flag vector. The recombinant pcDNA 3.1-Flag/Uncv plasmid was transiently expressed in HeLa cells. HeLa cell clones expressing fusion protein with molecular weight of about 95×103 were obtained. ConclusionsA recombinant eukaryotic expression plasmid of Uncv has been successfully constructed and expressed in HeLa cells. It may provide a foundation for further biological studies of Uncv gene.
