Abstract:ObjectiveTo observe the influence of different formulas of chow and different ages of rats on the success rate and characteristic pathological changes in the establishment of a rat model of diabetes mellitus. MethodsTwenty-six 3-week-old SD rats were divided into 3 groups:control group 1 (group N1,6 rats), model group 1 (M1 group, 10 rats), and model group 2 (M2 group, 10 rats). Twenty-six 5-week-old SD rats were divided into 3 groups:control group 2 (group N2,6 rats), model group 3 (group M3,0 rats), and model group 4 (group M4,0 rats). The groups M1 and M3 were fed with the formula 1 high fat chow, and the groups M2 and M4 were fed with the formula 2 high fat chow. The rat models were given streptozotocin (STZ) injection (35 mg/kg) once intraperitoneally after feeding with high fat chow for 4 weeks. The levels of FBG, FIN, TG, TC were detected successively. ResultsThe FBG level of 5-week-old SD rats reached a stable state 2 weeks after STZ injection, and kept on a high level. The rats fed with the formula 2 of high fat chow showed significant increase of food-intake and body weight, and had successfully induced insulin resistance. Conclusions5-week-old SD rats fed with the formula 2 of high fat chow and STZ injection present major pathophysiological manifestations of type 2 diabetes mellitus, and can be used as a good model of type 2 diabetes mellitus in relevant research.
