ObjectiveTo observe the expression of glutamine fructose-6-phosphate aminotransferase 1 (Gfat1) in the lung tissue of type 2 diabetic rats.MethodsSprague-Dawley rats were randomly divided into control group (n=18) and diabetic model group (n=28). Rats in the model group were fed with high-fat diet for 2 months, and then were injected intraperitoneally streptozotocin (STZ) at a dose of 15 mg/kg to generate diabetic models. Fasting blood glucose and body weight were recorded. The expression of Gfat1 mRNA in the lung tissue was detected by RT-PCR on the 2nd, 4th and sixth week after diabetes modeling, respectively. ResultsThe body weight of rats in the model group increased rapidly. Compared with that of the control group, body weight was significantly increased at the 28th, 42nd, 56th and 70th days from the modeling started (P<0.05). Fasting blood glucose (FBG) in the rats of model group after STZ injection was significantly higher (≥10.0),[JP+1] compared with that in the control group (P<0.01). Gfat1 expression in the rats of model group on the second week of diabetes modeling was lower than that in the control group, but the difference was not significant (P>0.05). Gfat1 expression of rats in the model group on the fourth week of diabetes modeling was higher than that in the control group, with a significant difference (P<0.05). Gfat1 expression in the rats of model group on the sixth week of diabetes modeling was higher than that in the control group, but the difference was not significant (P>0.05). ConclusionsType 2 diabetic rat model can be successfully established by feeding high-fat diet with intraperitoneal injection of streptozotocin. The level of Gfat1 expression is changed in the lung tissue at different periods of diabetes modeling.