ObjectiveTo develop a technique for establishing a rat model for end-to-side anastomosis of the common carotid artery (CCA). MethodsA surgical operation was performed to create an end-to-side anastomosis between the right and left CCA passing through the tunnel between the anterior cervicalmuscle group and the trachea in rats. ResultsAll the 10 Sprague-Dawley rats which underwent this procedure, survived the operation without postoperative neurological sequel. The success rate was 100%. The mean number of sutures required to complete the anastomosis was eight. The time spent to perform the anastomosis was (35±5)min. The anastomoses were almost 100% patent immediately and 3 months after the procedure. HE staining showed good anastomotic healing. ConclusionsA rat model for end-to-side anastomosis of the common carotid artery (CCA) has been successfully established. Careful operational micromanipulation and the protection of the small branches of nerves and blood vessels are the two key points for successful anastomosis. It may serve as a useful model for suture training in microvascular surgery.