ObjectiveTo analyze the resistin gene homology of humans and some laboratory animals, and provide a basis for the selection of ideal laboratory animals to carry out human disease and gene function studies. Methods To extract total RNA of adipose tissue from macaques, rats, mice and tree shrews. The RT-PCR products were analyzed with bi-directional sequencing. The sequences of resistin gene of human, pig and cattle were inquired in GenBank. Their ORF nucleotide and amino acid sequences were transfered to use the ORF Finder software, and analyzed their sequence homology. ResultsHuman resistin gene of nucleotide sequence homology was 95.4% with macaques, 65.4% with mice, 65.4% with tree shrews, 66.4% with rats, 81.0% with pigs and 81.0% with cattles. The amino acid sequence was 91.7% with macaques, 55.6% with mice, 55.6% with tree shrews, 53.7% with rats, 75.9% with pigs and 72.2% with cattles. The phylogenetic tree results showed a closer relationship between humans and macaques. ConclusionsBased on the resistin gene homology analysis results, it was found that macaques have a closer relationship with humans, and are best laboratory animals in studies of human resistin biological function and related diseases.