Objective To study the anti-fatigue effect of Nitraria seed oil in a mouse experiment. Methods Loaded swimming test was used to evaluate the anti-fatigue effect on the mice．Biochemical parameters such as the concentrations of lactic acid and glycogen were determined. ResultsThe swimming time in mice of the Nitraria seed oil group was significantly prolonged, and the serum lactic acid decreased by 40% than those of control mice. Conclusion Nitraria seed oil has some anti-fatigue effects.