Objective To investigate the variation of serum amino acids in diabetic mice in order to explore the application of metabolic profiling analysis in research of diabetes mellitus in mice. Methods Healthy male adult SPF Kunming mice were fed with high-fat diet for 4 weeks and then injected with streptozotocin (STZ). Fasting blood glucose (FBG) was monitored weekly after STZ injection. Blood samples of normal mice and model mice were collected at 4 weeks after STZ injection. The metabolic profiles were analyzed using pre-column derivatization HPLC. ResultsCompared with the control mice,the FBG, TG, and FFA were significantly higher than those in the model mice. The metabolic profile of the model mice was significantly different from that of the control mice. ConclusionsPattern recognition method is applied and demonstrates that four amino acid markers (arginine, phenylalanine, lysine, tuarine) are different between the normal and model mice. Amino acid metabolism profiling combined with pattern recognition may reflect to a certain extent the pathological changes of type 2 diabetic mice.