Abstract:Objective To establish a subcutaneous tumor model in nude mice by injection of human gastric carcinoma cell line BGC-823 cells in various concentrations and observe its biological characteristics．MethodsBGC-823 cell line was cultured and the cells were divided into four cell suspension groups:group1:5×107cells/mL, n=10; group 2:1×107cells/mL, n=10; group 3:1×106cells/mL, n=10; group 4:1×105cells/mL, n=10．Each of 40 mice was subcutaneously injected 0.2 ml BGC-823 cell suspension into the armpit. Food intake and activity status of tumor-bearing mice,tumor formation time,mental conditions, survival rate,tumor formation rate, growth and size of the tumors were observed．Immunohistochemical SP staining was adopted for assessment of the microvessel density (MVD). ResultsThere was no abnormity and death in the mice after injection of tumor cell suspension. The overall tumor formation rate was 100% in all groups except the group 4,and the average tumor appearing time was 3[KG-*2]-[KG-*9]7 days after injection of tumor cell suspension. The average tumor MVD was (123.26±31.57)/mm2. ConclusionsThe subcutaneous nude mouse models of human gastric carcinoma and the lowest concentration of tumor cells needed for the modeling are preliminarily established. It lays a foundation for experimental studies of gastric carcinoma.
