Objective Uterine epithelial cells were isolated from pregnant mice and cultured in vitro, and examined the effect of CD82 on the expression of integrin αV, β3, E-cadherin and β-catenin in the cells. Methods The uterine epithelial cells were primarily isolated from pregnant mouse uterus. The recombinant adenovirus containing mouse CD82 gene which had been constructed in our lab infected the uterine epithelial cells. Immunocytochemistry was used to detect the protein expressions of integrin αV, β3, E-cadherin and β-catenin in the uterine epithelial cells, which were infected with CD82 adenovirus or not. Results 1. The purity of primary cultured cells was (93.2 ± 0.6)%. 2. The transfection efficiency of CD82 recombinant adenovirus was (92±4.5)%. The adenoviral particles carrying CD82 gene indeed expressed CD82 gene and protein in the primary uterine epithelial cells after 24 hours or 48 hours. 3. The uterine epithelial cells of pregnant mice on d4 expressed integrin αV, β3, E-cadherin and β-catenin. 4. In contrast to the control group, when CD82 adenovirus infected cells, the uterine epithelial cells of pregnant mice on d4 increased the expression of integrin αV, β3 and β-catenin protein, had no significant changes of E-cadherin. Conclusions CD82 may have effect on the expression of integrin αV, β3 and β-catenin in mouse uterine epithelial cells before implantation.