FH/Wjd大鼠酒精性肝损伤模型探讨
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太行山道地、大宗药材综合利用技术及产业化开发项目(13042506Z).


Exploration of a FH/Wjd rat model of alcoholic liver disease
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    摘要:

    目的 对FH/W jd大鼠酒精性肝损伤模型进行探讨.方法 FH/W jd大鼠按体重随机分为饮水组和饮酒组,两组均自由饮食.16周后取血,检测血清ALT、AST、TBIL、TG、CHO;取肝脏,匀浆后检测TG、GSH;流式细胞仪检测肝细胞凋亡率;Western blot检测肝脏组织PPARα蛋白表达;肝脏HE染色观察组织病理学改变.结果 与饮水组比较,饮酒组两种性别FH/W jd大鼠血清TBIL、TG含量显著升高,饮酒组雌性大鼠血清ALT、CHO显著降低;饮酒组两种性别大鼠肝脏TG含量显著升高,GSH含量呈现降低趋势;饮酒组两种性别大鼠肝细胞凋亡率亦呈降低趋势;饮酒组肝脏PPARα蛋白表达明显上调;饮酒组组织病理学改变以小泡性脂肪变性为主.结论 长期自主摄入酒精可以造成FH/W jd大鼠肝脏损伤.

    Abstract:

    Objective To investigate the FH/Wjd rat model of alcoholic liver disease. Methods Thirty-six 16-18 week old SPF grade FH/Wjd rats (male:female=1:1) were used in this study. The rats were divided into two groups randomly by body weight: water intake group and alcohol intake group. The rats took water or alcohol freely. 16 weeks later, ALT, AST, TBIL, TG, CHO in the serum and TG, GSH in the liver homogenate were detected. The expression of PPARα protein in the liver tissue was detected by Western blot. The apoptosis rate of liver cells was assessed by flow cytometry. The pathological changes of liver tissue were examined using HE staining. Results Compared with the water intake group, the serum TBIL and TG were significantly increased in rats of both sexes of the alcohol intake group, moreover, ALT and CHO of the female rats in the alcohol intake group were significantly decreased. TG in the liver homogenate increased obviously, while GSH in the liver homogenate showed a decreasing tendency. Hepatocyte apoptosis in rats of both sexes in the alcohol intake group showed an increasing tendency. The PPARα protein expression was up-regulated obviously, and the main pathological change in the liver tissue was microvesicular fatty degeneration. Conclusion Spontaneous long-term alcohol intake can induce liver injuries in FH/Wjd rats.

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张彦芬,秘尧,何奇龙,王宏涛. FH/Wjd大鼠酒精性肝损伤模型探讨[J].中国实验动物学报,2014,22(5):59~62.

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  • 收稿日期:2014-06-08
  • 在线发布日期: 2014-10-30
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