南京军区南京总医院课题(2012052).
目的 研究外源绿色荧光蛋白(green fluorescent protein,简称GFP)基因在BALB/c绿色荧光裸鼠主要器官组织中的表达及其差异.方法 小动物成像系统和RT-PCR方法检测GFP的组织分布以及荧光表达水平情况.结果 经活体荧光影像系统观察及PCR方法检测发现GFP可以在裸鼠多个器官组织中表达,其中在胰腺、心脏、全脑、皮肤、睾丸中表达量较高.结论 外源绿色荧光蛋白可以在模型动物体内成功表达且稳定遗传,其中在胰腺组织中高表达.
Objective To study whether the green fluorescent protein (GFP) gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics. Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level. Results The GFP can be expressed in multiple tissues in green fluorescent nude mice. A higher expression was observed in the pancreas, heart, brain, and skin. Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas.
尤金炜,董敏,刘彪,梁磊,田迎,胡文娟,田小芸,方天,周森妹,赵志刚,恽时锋. GFP在绿色荧光裸鼠不同组织中的表达及分析[J].中国实验动物学报,2014,22(5):67~70.
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