Objective To establish a large animal (sheep) model to serve the experiments of domestic pulsatile ventricular assist device. Methods Three small-tail Han-sheep were anesthetized and the vein access and artery access were achieved. The cardiopulmonary bypass was established through left thoracotomy. Ventricular fibrillation was induced. An hole was made in the apex of left ventricle and the apex cannulation was sutured to it. The aortic cannulation was sutured to the descending aorta. The two cannulations were connected to the domestic pulsatile ventricular assist device (DPVAD) and the driver was turned on. The working of DPVAD and the conditions of the animals were observed. Results The DPVAD worked well and uni-directional blood flow was driven by positive and negative pressure. The left ventricle was unloaded and the blood pressure was raised up. Conclusion The establishment of sheep model of pulsatile ventrieular assist device may play important role for the research and development of DPVAD in our country.