山西农业大学科技创新项目(201204);国家自然科学基金(31172283);国家“863”项目(2013AA102506)
目的 HGF及其受体c-met是很多系统中间充质-上皮互相作用的主要的介导者,毛囊周期性生长是典型的间充质-上皮互作模型,为证实HGF/c-met信号在小鼠毛囊周期生长过程中是否发挥作用。方法 本实验运用免疫组织化学的方法对HGF/c-met在ICR小鼠毛囊生长期、退化期和休止期进行组织定位。结果 HGF主要位于真毛乳头和皮脂腺,c-met主要位于毛基质、根鞘部和表皮。HGF及其受体在小鼠毛囊生长期表达量达到最高值,在退化期基本不表达,在休止期-生长期过渡时HGF及其受体表达量均上升。结论 HGF/c-met信号在ICR小鼠毛囊生长过程中起调控作用。
Objective HGF and its receptor (c-met) are principal mediators of mesenchymal-epithelial interaction in several different systems. Moreover, hair follicle is a model of mesenchymal-epithelial interaction. The aim of this study was to explore whether HGF/c-met signal plays a role in the control of hair follicle growth cycle. Methods To examine the localization of HGF/c-met in anagen, catagen and telogen in the hair follicle of ICR mice. Results HGF was mainly located in the dermal papilla and sebaceous gland, and c-Met in the hair matrix, root sheath and epidermis. Both HGF and c-met expressions peaked during anagen, not found in catagen, and increased during telogen-anagen phase. Conclusions Our results demonstrate a regulatory role of HGF and c-met in the control of hair follicle growth in ICR mice.
于秀菊,高淑媛,程笳琪,赵兵令,赫晓燕,董常生. HGF/c-met在小鼠毛囊生长周期中的表达[J].中国实验动物学报,2015,23(4):406~409.
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