Objective To study the effect of low frequency electrical stimulation on hormone levels in organs of Chinese tree shrews after death. Methods Giving Chinese tree shrews low frequency electrical stimulation.At 0 h, 3 h, 6 h, 12 h, 18 h, 24 h, 36 h and 72 h after death, the thyroid, liver, spleen were taken,and the levels of endothelin (ET), atrial natriuretic factor(ANF), thromboxane (TX) were determined by RIA method.At 0 h after death, midbrain ventral tegmental area (VTA) of Chinese tree shrews was taken to detect the c-fos expression. Results After electrical stimulation, ET, ANF, TX levels in the cadaver organs and VTA c-fos expression of Chinese tree shrews were significantly increased than in the control group. The contents were decreasing with the time after death. Conclusions Low frequency electrical stimulation can induce the synthesis and release of hormones in organs and c-fos expression in brain tissue of Chinese tree shrews.