Objective To investigate the genetic patterns of iris pigmentat mutation in the white hair black eye (WHBE) rabbits. Methods To construct six two-generation families between WHBE rabbit and Japanese white rabbit, and the quantity of individuals had different eye colours in F1 and F2 generations were recorded and analyzed.Results χ² test showed there was no significant difference between observed values and expected values in the mode of autosomal dominate inheritance (P>0.05), while there were significant differences between observed values and expected values in the autosomal recessive inheritance and sex-linked genetic pattern(P<0.05).Conclusions Iris pigmentat mutation in WHBE rabbit has a monogenic character due to autosomal dominant mutation.