Objective To analyze the Alb-cre/DTR mouse phenotype, and establish a model of induced liver damage to serve basic researches of liver diseases. Methods The introduced Alb-cre and DTR mice were crossed to obtain Alb-cre/DTR mice and the genomic DNAs were extracted from the tail tissue of the mice for genotying by PCR. Diphtheria toxin was intraperitoneally(i.p.)injected into the Alb-cre/DTR mice, then the body weights were monitored and the sera were collected for the detection of serum ALT and AST levels. Results By crossing Alb-cre and DTR mice we obtained the Alb-cre and DTR double transgenic mouse. The intraperitoneal injection of diphtheria toxin in a dose of 0.625 ng/g body weight significantly induced liver injury in these mice, as showed by the elevated levels of ALT and AST, the gross appearance of liver damage and the pathological changes such as necrosis in the liver tissue. Conclusions We have obtained a novel mouse strain of Alb-cre/DTR by crossing Alb-cre and DTR mice. Liver damages in those Alb-cre/DTR mice can be induced by injection of diphtheria toxin. This established mouse model of inducible liver damage is a useful platform for the studies of liver damage and recovery, as well as liver transplantation.