Objective To verify the detection ability of experimental animal quality detection laboratories in China for Staphylococcus aureus. Methods The testing samples for Staphylococcus aureusdetection were prepared by bacterial culture, homogeneity test and stability test, according to the study plan approved by CNAS. Then the samples and operation instruction were sent to the participant laboratories. The detection reports from these laboratories should be submitted before the deadline expires, and the collected data were summarized and analyzed. Results There were 28 laboratories which joined to this test plan. Among them 22 laboratories(78.57%) achieved satisfactory test results, and six laboratories(21.43%) had unsatisfactory test results. 27 Laboratories used the national standard detection assay, while only one laboratory used PCR assay. Conclusions Most of experimental animal quality testing laboratories in China have sufficient proficiency in detection of Staphylococcus aureus. The obtained information are very helpful for the laboratory ability verification testing in future.