上海市科技创新行动计划(14140900600,11140901000);上海市科委研发平台专项(15DZ2292400)
目的 探讨肝螺杆菌(Helicobacter hepaticus,H.hepaticus)感染对小鼠骨髓源树突状细胞(DC)表面分子形态和机体免疫应答的干扰。方法 以H.hepaticus(ATCC 51450)灌饲SPF级BALB/c雄性小鼠,于末次接种后5个月体外分离培养骨髓DC,经粒细胞-单核细胞集落刺激因子(GM-CSF)、白细胞介素-4(IL-4),刺激DC增殖、分化,流式细胞仪分析DC细胞表面分子CD11c、CD40、CD80、MHCⅡ的表达率。此基础上,用新城疫病毒(NDV)ZJ1株人工接种实验组和对照组小鼠,每周测定NDV血清抗体效价,比较抗体产生的差异。结果 实验组MHC Ⅱ和CD40分子的表达率高于对照组。NDV抗体水平第1周实验组略低于对照组;第2~5周内实验组小鼠的抗体水平均高于对照组,差异有显著性;第6周两组小鼠血清抗体均呈下降趋势,差异无显著性。结论 H.hepaticus感染对小鼠骨髓DC成熟有促进作用,能提高MHC Ⅱ和CD40表达水平,可促进BALB/c小鼠产生抗NDV的抗体水平。
Objective To determine the interference effect of H.hepaticus infection on the functional characteristics of dendritic cell (DC) surface molecules and immune response in mice. Methods Male BALB/c mice were inoculated with H.hepaticus (ATCC 51450).Murine bone marrow-derived dendritic cells (DC) were isolated and co-cultured which were stimulated by GM-CSF and IL-4 at the fifth month after the last inoculation. Then the DCs were subjected to FACS analysis for surface markers (CD11c, CD40, CD80 and MHCⅡ) detection.On this basis, virus suspension of Newcastle disease virus(NDV) ZJ1 strain was inoculated into the mice. Serum was collected for detection of the NDV antibody titer in serum weekly to explore the difference of antibody titer between the two groups.Results The expression rates of CD40 and MHCⅡ on the mouse DCs in experimental group were higher than that in the control group. The NDV antibody titer of experimental group was slightly lower than that in the control group in the first week. During the 2nd to 5th weeks, the titer was higher than that in the control group, with a very significant difference. In the 6th week, the titer of both the two groups tended to fall.Conclusions H.hepaticus infection can promote bone marrow DC maturation in mice, stimulate the expression rates of MHC Ⅱ and CD40, and enhance the NDV antibody levels.
冯洁,张泉,谢建云,魏晓锋,高诚.肝螺杆菌感染对BALB/c小鼠免疫应答干扰的初步研究[J].中国实验动物学报,2016,24(3):304~308.
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