Objective To establish a breeding method of Myodes rufocanus in the laboratory, collect their growth and reproduction data, and provide a basis for carrying out the experimental animalization.Methods Wild Myodes rufocanus caught in the Moranbong woodland were brought back to the laboratory. They were bred artificially in a large hard wall rodent negative pressure isolator. Their growth and reproduction data were recorded for evaluating the results of breeding. Results The Myodes rufocanus were successfully bred in the laboratory. The pregnancy rate was 54.55%.The average pregnancy length was 20.4 days(8 to 22 days). During one breeding period,they gave birth 2.9 times on average. The maximum number of births was 7 times,far more than the number tested under field conditions.The average litter size was 4.3±1.22. The highest litter number of a single nest was 8. The weaning rate of pups was 94.8%. The growth and development of pups were good. Conclusions The breeding method for Myodes rufocanus is established. The growth and reproduction data are tested too. The results of our study laid a foundation for the experimental animalization of Myodes rufocanus.