Objective To investigate the growth curve, breeding rate, and blood physiological and biochemical parameters in Npc1 gene mutant mice (Npc1^-/-) for providing theoretical evidence in research on Niemann-Pick disease type C1 (NPC1) patient. Methods 1) The body mass of Npc1^-/-, Npc1^+/-, and Npc1^+/+ mice (n=120; 60♀, 60♂) was measured from 0 to 77 days; (2) As Npc1^-/- mice were born only by the mating Npc1^+/- mice, the breeding rate of Npc1^+/- mice was counted here from the 1st to 4th generation; (3) The blood physiological and biochemical parameters were measured on both Npc1^-/- and Npc1^+/+ mice at 60 days. Results 1) Compared with the wild type controls,the body weight of Npc1^-/- mice was progressively increased up to 7 weeks and then decreased, and died around 11 weeks. The body weight of the Npc1^+/- and Npc1^+/+ mice was increased as time went on. After 4 weeks, the male mice showed a higher weight gain than the females; (2) The generations of Npc1^+/- mice had no significant difference in mating-parturition interval, litter size, weaning litter and the number of male and female (P>0.05), but the weaning rate of the 2nd generation was significantly higher than that of the 1st generation (P<0.05); (3) The hematological parameters showed a significant difference only in mean corpuscular hemoglobin (MCH) and mean peroxidase index (MPXI) between the Npc1^-/- and Npc1^+/+ mice (P<0.05). No significant difference was found in other hematological parameters (P>0.05). Among the biochemical parameters, aspartate aminotransferase (AST), glucose (GLU), lactate dehydrogenase (LDH), potassium (K) and copper (Cu) had a significant difference between the Npc1^-/- and Npc1^+/+ mice (P<0.05). Conclusions 1) The growth curves of Npc1^-/-, Npc1^+/-, and Npc1^+/+ mice are different due to different genotype and sex; (2) The reproduction rates of Npc1^+/- mice have no significant difference among different generations; (3) The blood physiological parameters (MCH, MPXI) and biochemical parameters (UREA, AST, GLU, LDH, K, Cu) are significantly different between Npc1^-/- and Npc1^+/+ mice.