Location and expression of vitamin D receptor in the duodenum of C57BL/6 mice at different developmental stages
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摘要:
目的 为探讨维生素D受体（vitamin D receptor，VDR）在C57BL/6小鼠十二指肠中的定位与表达，研究维生素D系统与肠道发育过程的相关性。方法 利用实时定量PCR （quantitative PCR，qPCR）、苏木精伊红染色、免疫荧光染色、免疫印迹（Western blotting，WB）的检测技术，对C57BL/6小鼠十二指肠中VDR定位表达做定性与定量的分析。结果 VDR的mRNA在C57BL/6小鼠十二指肠组织中的表达在其出生的第21天达到峰值。此外，VDR主要分布在C57BL/6小鼠十二指肠上皮细胞和平滑肌细胞的胞质中。对组织样品的WB检测显示，C57BL/6小鼠十二指肠发育过程中，VDR没有发生明显的核转位。结论 从分子生物学角度分析了VDR在C57BL/6小鼠十二指肠不同生长发育阶段的表达规律，为了解和完善VDR在肠道中的作用奠定理论基础。
Abstract:
Objective To investigate the relationship of vitamin D with the intestinal development and study the expression of vitamin D receptor (VDR) in the duodenum of C57BL/6 mice at different developmental stages. Methods Quantitative PCR (qPCR), histology using H&E staining and immunofluorescence staining, and Western blotting (WB) were performed to elucidate the expression of VDR in mice intestine at different growth and developmental stages. Results The peak of VDR mRNA expression reached on 21 d. The pathological result showed that VDR mainly distributed in the cytoplasm of epithelial cells and smooth muscle cells in the mouse duodenum. WB result indicated that there was no nuclear translocation of VDR protein in the mouse duodenum. Conclusions This study demonstrates the regularity of expression of VDR in the mouse duodenum during its development, and contributes to understanding the function of VDR in the intestines.
