诸氏鲻虾虎鱼封闭群遗传质量评价方法的建立
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广东省实验动物监测所,广东省实验动物重点实验室,广州 510663


Evaluation methods to establish the genetic quality of closed colony Mugilogobius chulae
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Key Laboratory of Guangdong Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China

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    摘要:

    目的 分析诸氏鲻虾虎鱼封闭群和野生群的遗传差异,建立诸氏鲻虾虎鱼封闭群遗传质量评价方法。 方法 利用 20 个微卫星标记对诸氏鲻虾虎鱼野生群和封闭群进行 STR 分型,计算群体间遗传杂合度和遗传分化状况,并分析样本量对遗传参数的影响。 结果 诸氏鲻虾虎鱼封闭群和野生群在 20 个微卫星位点的平均无偏期望杂合度(He)分别为 0.6927 和 0. 7162。封闭群与野生群间遗传分化较小,且当样本量达到 30 尾以上后,平均等位基因数趋于稳定。 结论 遗传杂合度是否在 0. 5 ~ 0. 7 之间可较好将诸氏鲻虾虎鱼封闭群与野生群区分, 适于作为诸氏鲻虾虎鱼封闭群遗传质量合格与否的判定指标。

    Abstract:

    Objective To analyze the genetic differences between closed colony and wild population of Mugilogobius chulae, and to establish a genetic quality evaluation method of closed colony M. chulae. Methods Twenty microsatellite markers were selected to detect heterozygosity, genetic differentiation, and the relationship between sample size and the genetic parameters of closed colony and wild population of M. chulae. Results The unbiased expected heterozygosity (He) of closed colony and wild population of M. chulae were 0. 6927 and 0. 7162, respectively. Analysis of genetic differentiation showed little difference between closed colony and wild population. Analysis of the relationship between sample size and genetic parameters showed that the mean number of alleles tended to be stable at a sample size of 30. Conclusions Heterozygosity in the range of 0. 5 – 0. 7 can distinguish closed colony strains of M. chulae from wild population, and would be suitable for determining the genetic quality of closed colony M. chulae.

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蔡磊,李建军,余露军,黄韧.诸氏鲻虾虎鱼封闭群遗传质量评价方法的建立[J].中国实验动物学报,2020,28(1):10~16.

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  • 收稿日期:2019-11-28
  • 在线发布日期: 2020-04-01
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