1.四川大学华西第二医院放射科,出生缺陷与相关妇儿疾病教育部重点实验室,成都 610041; 2. 四川大学华西医院放射科,成都 610041
1.Department of Radiology, West China Second Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Chengdu 610041, China. 2. Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041
目的 探索经兔耳中央动脉穿刺入路,利用球囊损伤兔颈总动脉内膜联合高脂饲料喂养,构建兔颈总动脉粥样硬化模型的新方法并评价可行性。 方法 将兔经高脂饲料预喂养 4 周后,经兔耳中央动脉穿刺入路, 利用球囊损伤右侧兔颈总动脉内膜,再经高脂饲料喂养 8 周。 利用磁共振仪和病理组织学观察兔颈总动脉病变。 结果 本研究构建兔颈总动脉粥样硬化动物模型的成功率为 85%。 在磁共振图像上,实验组颈总动脉血管壁增厚,明显强化,且实验组动脉粥样硬化斑块的信号强度和信噪比明显高于自身对照组。 实验组颈总动脉血管壁内膜呈现动脉粥样化斑块的病理表现,而自身对照兔颈总动脉血管壁内膜正常。 结论 通过兔耳中央动脉穿刺入路,行球囊损伤兔颈总动脉内膜联合高脂饲料喂养的方法成功构建了兔颈总动脉粥样硬化斑块模型,该方法具有创伤小、操作简单、重复性较强、造模用时短以及成模率高等优点。球囊损伤颈总动脉内膜采用了独创的兔耳中央动脉穿刺入路,为构建兔颈总动脉粥样硬化斑块模型开辟了新思路。
Objective To establish a rabbit model of carotid atherosclerotic plaque by puncturing from central artery of rabbit ear and high-fat diet. Methods All rabbit were fed with high-fat diet for 4 weeks. By puncturing from central artery of rabbit ear, the balloon was inflated to injury intima of carotied artery and then followed with 8 weeks of high-fat diet. The right common carotid artery which damaged by balloon catheter were defined as experimental group and the left common carotid artery as self-control group. MRI and histopathology were used to evaluate common cervical artery lesions in rabbits. Results The success rate of modeling was 85. 0%. On MRI scan image, the wall of carotid artery in the experimental group was thickened and obviously strengthened, while there was no thickening of carotid artery wall in the control group, and no obvious enhancement was observed. In the experimental group, the intima of the common carotid artery wall presented the pathological manifestations of atherosclerotic plaques. In the control group, the inner membrane of carotid artery wall was normal. Conclusions A rabbit model of carotid atherosclerosis was successfully constructed with balloon injury by puncturing from central artery of rabbit ear accompanied high-fat diet. The modified method have advantages of small trauma, simple operation, strong repeatability, short modeling time and high success rate.
叶鹏飞,郭应坤,张怡,沈梦婷,李湘,陈林,徐婷,文凌仪.构建兔颈总动脉粥样硬化斑块模型的新方法及评价[J].中国实验动物学报,2021,29(3):301~306.
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