Objective To establish a rat model of chronic hyperuricemia renal damage, and to provide a model tool for the development of drugs for anti-chronic hyperuricemia renal damage. Methods Forty male sprague-dawley (SD) rats were randomly divided into five groups: normal group, group A (fed with 2% oteracil potassium + 12% yeast extract + 86% common feed), group B ( fed with 0. 15% adenine + 10% yeast extract + 89. 85% common feed), group C (100 mg / kg adenine + 1500 mg / kg oteracil potassium, intragastric administration in the morning and evening), and group D (50 mg / kg adenine + 1500 mg / kg oteracil potassium, intragastric administration every morning). The observation time was 5 weeks. The body weight, serum uric acid, creatinine, urea nitrogen, and other indicators of rats in each group were detected every week. After 5 weeks, the ratio of bilateral kidney weight to body weight as well as a pathological section of kidney were observed. Results The weight of rats decreased, the ratio of kidney weight to body weight increased, serum creatinine and urea nitrogen all increased, kidney color changed significantly, and kidney damage was identified by hematoxylin-eosin (HE) staining and urate staining in group C compared with the normal group. Conclusions In conclusion , intragastric administration of 100 mg / kg adenine + 1500 mg / kg oteracil potassium in the morning and evening was the best way to establish a rat model of chronic hyperuricemia renal damage.