1.成都中医药大学针灸推拿学院,成都 610072; 2. 成都中医药大学研究生院,成都 610072
1.Acupuncture and Tuina College, Chengdu University of Chinese Medicine, Chengdu 610072, China. 2. Graduate School, Chengdu University of Chinese Medicine, Chengdu 610072
肠易激综合征(irritable bowel syndrome,IBS)是临床常见的功能性肠病,以反复发作的腹痛、排便习惯改变及大便性状改变为主要临床特征。 目前 IBS 病因及发病机制虽尚未明确,但内脏高敏性是其重要的病理特征之一被广泛接受,同时内脏高敏也是 IBS 患者腹痛、腹胀等临床症状的重要病因。因此,成功模拟 IBS 患者内脏高敏感性成为了 IBS 相关动物实验的一个重要环节。建立一种客观稳定的内脏高敏动物模型亦变得尤为重要。结直肠扩张法(colorectal distention,CRD)以其模型内脏高敏感性持续稳定而应用广泛。 CRD 通过持续的机械刺激因素造成大鼠内脏高敏感,并且结直肠无明显病理改变。通过对 CRD 的操作、存在问题及应对方法进行讨论,总结各实验造模方法在实验对象、球囊种类、扩张方式等相关因素方面的差异,为使用 CRD 进行内脏高敏模型制作的研究者们提供参考,为建立客观、可靠且重复性好的内脏高敏动物模型提供研究思路。
Irritable bowel syndrome ( IBS) is a common intestinal disease characterized by recurrent abdominal pain and changes in the feces and / or defecatory habits. However, although the etiology and pathogenesis of IBS remain unclear, visceral hypersensitivity is widely accepted to be an important pathogenetic factor. Therefore, it is important to be able to study an appropriate animal model of visceral hypersensitivity. Colorectal distension (CRD) is widely used for this purpose because it induces persistent and stable visceral hypersensitivity through continuous mechanical stimulation that is not associated with obvious pathological changes in the rectum, which is consistent with the pathological characteristics of IBS. In the present paper, we discuss the surgical procedure, the limitations of CRD, the various experimental devices and expansion method used, and other related factors in order to provide a reference for researchers who wish to use CRD-based models of visceral hypersensitivity.
蒋慧灵,郑倩华,赵映,张薇,侯雨君,谭玉,李瑛,蔡定均.结直肠扩张法诱导大鼠内脏高敏感模型制作概述[J].中国实验动物学报,2021,29(4):528~534.
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