Zebrafish has become a widely used model organism to study vertebrate development, genetic mechanisms of diseases, and drug toxicology. There is high similarity in the morphology, physiology, functions, and gene expression of zebrafish and human eyes. Furthermore, zebrafish embryos are easy to obtain, develop rapidly, and easily observed and manipulated. Therefore, zebrafish has become a good animal model for human ophthalmic diseases and provide new insights to further explore the pathogenesis of diseases and treatment method . This review introduces the ocular characteristics of zebrafish, the application of zebrafish animal models in ophthalmic diseases, and evaluation of ophthalmic drug efficacy and ocular toxicity of drugs.