1. 上海中医药大学附属曙光医院,上海 201203;2. 上海中医药大学肝病研究所,上海 201203;3. 肝肾疾病病证 教育部重点实验室,上海 201203
PING Jian
1.Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. 2. Institute of Hepatology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203. 3. Key Laboratory of Liver and Kidney Diseases, Ministry of Education, Shanghai 201203XU Lieming
1.Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. 2. Institute of Hepatology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203. 3. Key Laboratory of Liver and Kidney Diseases, Ministry of Education, Shanghai 2012031.Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. 2. Institute of Hepatology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203. 3. Key Laboratory of Liver and Kidney Diseases, Ministry of Education, Shanghai 201203
目的 观察 C57BL/ 6 背景的 Mdr2 基因敲除小鼠自发肝肿瘤形成情况。 方法 (11. 3 ± 4. 2)周龄 Mdr2 基因敲除 C57BL/ 6-Abcb4tm1 小鼠 9 只和野生型 C57BL/ 6 小鼠 5 只,连续饲养 65 周后处死小鼠,留取血清及肝标本。 检测血清 ALT、AST、AFP 水平,肝组织石蜡切片做 HE、天狼猩红染色,免疫组织化学检测肿瘤及肿瘤旁组织 CK-7、CK-19 表达情况。 结果 9 只 Mdr2 基因敲除小鼠均自发形成肝肿瘤,血清 ALT、AST、AFP 水平均显著高于野 生型小鼠(P< 0. 01),Mdr2 基因敲除小鼠肝肿瘤 CK-7、CK-19 染色均为阴性。 结论 Mdr2 基因敲除小鼠连续饲养 至(76. 3 ± 4. 2)周龄时均自发形成肝肿瘤,其病理组织分型为肝细胞癌。
Objective To observe spontaneous liver tumor formation in Mdr2 knockout mice with C57BL/ 6 background. Methods Five wild type C57BL/ 6 mice and nine Mdr2 knockout C57BL/ 6-Abcb4tm1 mice aged ( 11. 3 ± 4. 2)weeks were sacrificed after 65 weeks of continuous feeding. Serum and liver samples were collected and serum ALT, AST, and AFP levels were measured. Paraffin-embedded sections of liver tissue were stained with HE and Sirius red. CK-7 and CK-19 expression in tumor and adjacent tissues was detected by immunohistochemistry. Results Nine Mdr2 knockout mice formed liver tumors spontaneously. Serum ALT, AST, and AFP levels were significantly higher than those in wildtype mice(P< 0. 01). CK-7 and CK-19 expression was negative in Mdr2 knockout mice. Conclusions The Mdr2 knockout mice formed liver tumors spontaneously when continuously fed to ( 76. 3 ± 4. 2 ) weeks of age, and their pathological classification was hepatocellular carcinoma.
李萌,平键,徐列明. Mdr2 基因敲除小鼠建立原发性肝癌模型观察[J].中国实验动物学报,2022,30(8):1058~1063.
复制
