The Langendorff ex vivo heart perfusion model is a widely used technique used for investigating myocardial ischemia?reperfusion injury (MIRI) because of its notable advantages of high reproducibility, ease of drug administration, and low technical requirements. This model excludes confounding influences from other organ systems, neural pathways, and circulating factors, making it an ideal choice to study MIRI?related diseases. In this review, we provide a comprehensive assessment of the Langendorff perfusion technique, encompassing its procedural steps, animal selection criteria, perfusion modes, choice of perfusion buffer, relevant assessment indices, methods of inducing ischemia, and considerations for ischemia and reperfusion time intervals. We discuss the strengths and limitations of this model to aid researchers in its proper application in studying MIRI. By analyzing the relevant literature, this review serves as a valuable resource to enhance understanding and implementation of the Langendorff perfusion model in cardiac research.