Objective To supplement basic research data and explore the value of tree shrews as an experimental animal in heart research. Methods Ten adult tree shrews, half male and half female, were selected to perform limb lead electrocardiograms. The common parameters of echocardiography of rats were used to assess the echocardiography of tree shrews. The cardiac structure and functions of tree shrews were assessed. HE staining was used to observe heart morphology. Results Tree shrew showed a sinus rhythm and electrocardiographic parameters (P wave, QRS complex, and T wave) similar to human normal electrocardiographic patterns. The heart of tree shrews consisted of four chambers, including two atria and two ventricles. Early diastolic filling (E) and atrial kick (A) waves were detected. The left ventricular wall thickness of tree shrews was measured by B? and M?type echocardiography, and indicators, such as ejection fraction (EF) and fractional shorting ( FS), were obtained. The cardiac wall was divided into the epicardium, myocardium, and endocardium. The ventricular wall consisted of three layers: superficial ( subepicardial ), middle, and deep (subendocardial). Conclusions Tree shrew have the potential to be used as heart disease models for scientific research.