基于数据挖掘的扩张型心肌病动物模型研究
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河南中医药大学,郑州 450046


Data mining-based animal model for dilated cardiomyopathy
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Henan University of Chinese Medicine, Zhengzhou 450046, China

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    摘要:

    目的 梳理国内外扩张型心肌病(dilated cardiomyopathy,DCM)动物模型的动物种类、造模方法、评价指标,为提高造模成功率和未来建立中医药特色的DCM 动物模型提供建议,为DCM 动物模型建立标准提供参考。 方法 检索近10 年扩张型心肌病动物模型的文献,收集整理相关报道,归纳总结实验动物相关参数(种类、性别、体重等)、造模方式、造模周期、阳性对照药种类及周期、模型评价指标等,并进行分析。 结果 共纳入128 篇文献。多采用蒽环类药物阿霉素诱导SD 大鼠和Wistar 大鼠制备扩张型心肌病动物模型,周期在30 ~ 60 d;通常应用超声心动图和苏木精-伊红(HE)染色检查模型复制是否成功;在含有阳性对照药的研究中,应用最多的是卡托普利,其次是卡维地洛;未有研究涉及明确的中医证候造模。 结论 扩张型心肌病动物模型目前造模方法较多,但缺少统一的评价标准,因此模型评价指标建议综合影像学、病理、生化、一般状况等结果;其次,国内对前沿遗传基因型动物研究较少,药物诱导方法缺乏造模药物的标准用量,未来扩张型心肌病动物模型造模方法和评价体系还有待进一步完善;最后,相较于心力衰竭动物模型,缺乏相关证候造模的表述,扩张型心肌病中医药动物模型还需进一步发展。

    Abstract:

    Objective To review the animal species, modeling method, and evaluation indexes of dilated cardiomyopathy (DCM) animal models at home and abroad to provide suggestions for improving the success rate of establishing DCM animal models with Chinese medicine characteristics, and to provide a reference to establish future DCM animal models. Methods The literature on animal models of dilated cardiomyopathy in the past 10 years was searched, and relevant reports were collected and summarized. Relevant parameters of experimental animals (species, sex, and body weight, et al), modeling method, modeling cycles, positive control drugs and cycles, and model evaluation indexes were summarized and analyzed. Results A total of 128 reports were included. Animal models of dilated cardiomyopathy were mostly prepared in SD and Wistar rats using anthracycline and adriamycin, with a cycle of 30 ~ 60 d. Echocardiography and pathological HE staining were usually applied to assess the success of model establishment. Among the studies containing positive control drugs, captopril was the most often used, followed by carvedilol. No studies involved explicit TCM evidence-based modeling. Conclusions Although many method are used to model dilated cardiomyopathy in animals, unified evaluation criteria are lacking. Therefore, it is recommended to integrate the result of imaging, pathology, biochemistry, and general conditions into the evaluation index of the model. Few studies on animals with frontier genetic genotypes exist in China, and standard dosages of modeling drugs for drug induction method are limited. Therefore, future modeling method and evaluation systems of dilated cardiomyopathy animal models need to be improved. Compared with animal models of heart failure, there is a lack of relevant evidence modeling, and the animal models of dilated cardiomyopathy in Chinese medicine require further development.

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葛君玺,秦格,谢逸轩,苗明三,白明.基于数据挖掘的扩张型心肌病动物模型研究[J].中国实验动物学报,2023,31(9):1167~1176.

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  • 收稿日期:2023-02-16
  • 在线发布日期: 2023-12-29
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