Abstract:Micro-computed tomography (Micro-CT) is a non-invasive technology that is widely used in animal experiments to assist in the detection of bone, lung, oral, metabolic, middle and inner ear diseases, as well as tumors, and in other animal disease models. The technique can provide diverse scientific and reliable imaging data for animal experiments and has accordingly become an indispensable experimental method in animal experiments. In this review, we introduce the imaging principles of Micro-CT, review its application in the study of animal disease models, summarize the limitations of Micro-CT technology, and consider its future prospects.