Objective Establishment and evaluation of a mouse model of aldosterone-induced multi-organ damage. Methods Twenty mice were randomly divided into four groups, with five mice in each group: a blank control group (0 μg / (kg·d)), a low-dose aldosterone group (150 μg / ( kg·d))), a medium-dose aldosterone group (300 μg /(kg·d)), and a high-dose aldosterone group ( 450 μg / ( kg·d)). Aldosterone-containing osmotic minipumps were surgically implanted under the skin, and aldosterone was infused for 4 weeks to establish the aldosterone-induced damage model. The body weight and blood pressure of the mice were recorded weekly. After the 4 week modeling period, the mice were euthanized, and their tissues were collected for observation and analysis of blood pressure and histological morphology of various organs. Results ( 1) After 4 weeks of aldosterone infusion, the serum aldosterone levels were significantly increased in the medium-dose and high-dose aldosterone groups, but not in the low-dose aldosterone group. (2) After the implantation of osmotic minipumps, the systolic blood pressure was significantly increased in the low-dose, medium-dose, and high-dose aldosterone groups during the second and third weeks, but decreased in all these groups during the fourth week. (3) The kidney and heart in the low-dose, medium-dose, and high-dose aldosterone groups showed varying degrees of damage, interstitial edema, collagen deposition, and fibrotic lesions. The liver in the low-dose aldosterone group showed a small amount of collagen deposition, while the medium-dose and high-dose aldosterone groups showed varying degrees of hepatocyte damage, collagen deposition, and fibrotic lesions. Conclusions Aldosterone can induce multi-organ damage in mice. Under this modeling method, organ damage is mainly manifested as edema, collagen deposition, and fibrotic lesions.