1. 江西中医药大学实验动物科技中心,南昌 330004;2. 江西金贝农业发展有限公司,江西新余 338000
1. Laboratory Animal Science and Technology Center of Jiangxi University of Chinese Medicine, Nanchang 330004, China; 2. Jiangxi Jinbei Agricultural Development Co., Ltd, Xinyu 338000, China
目的 探究江香薷籽油(Mosla chinensis seed oil,MCSO)对果蝇的生理行为和抗氧化指标的影响。 方法 将1日龄野生型W1118黑腹果蝇(Drosophila melnogaster,D. melanogaster)分为对照组、0.25%、0.5%、1%、2%和4%剂量组,并设雌雄分组。对照组喂食基础培养基,各剂量组分别喂食添加了0.25%、0.5%、1%、2%和4%浓度的MCSO培养基,采用攀爬实验探究最佳给药浓度和给药时间。再将果蝇分为空白组、模型组和给药组,模型组果蝇通过夜间重复光照刺激法剥夺果蝇睡眠,建立失眠模型。采用食欲实验、攀爬实验、应激实验、嗅觉记忆实验和检测果蝇睡眠-觉醒节律探究MCSO对果蝇生理行为的影响;采用酶联免疫吸附实验检测果蝇体内超氧化物歧化酶(super oxidase dismutase,SOD)、过氧化氢酶(catalase,CAT)活性和丙二醛(malondialdehyde,MDA)含量。 结果 MCSO能够增强30日龄果蝇的运动能力(P<0.01);提高SOD、CAT活性(P<0.01)、降低体内MDA含量(P<0.01);改善衰老果蝇的嗅觉记忆能力。睡眠剥夺后雌性果蝇模型组夜晚睡眠时间减少(P<0.05),雄性果蝇模型组夜晚睡眠时间减少(P<0.01),喂食MCSO后,睡眠剥夺的雌性果蝇夜间睡眠时间延长(P<0.05),雄性果蝇夜间睡眠时间延长(P<0.01)。 结论 MCSO对果蝇具有一定的体内抗氧化能力,能延长睡眠剥夺果蝇的夜间睡眠时间以及提高果蝇的嗅觉记忆力。
Objective To investigate the effects of MCSO on physiological behavior and antioxidant index in D. melanogaster. Methods One-day-old wild type D. melanogaster was divided into control group, 0.25%, 0.5%, 1%, 2% and 4% dose groups, as well as male and female groups. The control group was exposed to the base medium, and each dose group was exposed to the MCSO medium added with 0.25%, 0.5%, 1%, 2% and 4% concentrations, respectively. The optimal dosage concentration and time of administration were investigated by climbing experiment. Then the flies were divided into control group, model group and MCSO group. The model group was established by depriving the flies of sleep through repeated nocturnal light stimulation. Period of drug treatment, appetite test, negative geotaxis ability test, stress test, olfactory memory test, and sleep-wake rhythm detection were used to explore the effects of MCSO on their physiological behavior. The activities of super oxidase dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Results MCSO enhanced the locomotory ability of 30-day-old D. melanogaster (P<0.01), increased the activity of SOD and CAT (P<0.01), and decreased the concentration of MDA (P<0.01). Improve olfactory memory of senile fruit flies. After sleep deprivation, the night sleep time of female Drosophila model group was reduced (P<0.05), and that of male Drosophila model group was reduced (P<0.01). After feeding MCSO, the night sleep time of female drosophila model group was extended (P<0.05), and that of male drosophila model group was extended (P<0.01). Conclusions MCSO had a certain antioxidant effect, prolonging the sleep time and improving the olfactory memory of sleep-deprived Drosophila.
徐梦婷,朱钰晨,苏丹,宋永贵,张文凯,徐磊,马秋婷,刘志勇,郭绍勇.江香薷籽油对果蝇睡眠、嗅觉能力和抗氧化指标的影响[J].中国实验动物学报,2024,32(9):1182~1190.
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