Sarcopenia is an age-related skeletal muscle degenerative disease. Physiologically aging mice are the most commonly used animal model for studying sarcopenia. As sarcopenia is characterized by decreased skeletal muscle mass and reduced muscle strength, exercise performance as a reflection of muscle function is widely used to evaluate sarcopenia.Methods of evaluating the motor function of sarcopenia mouse models are generally designed based on muscle endurance, muscle strength, coordination, and balance. The method include tests such as treadmill exhaustion, voluntary wheel use, grip strength, horse grid, bars and balance beam tests. By collating recent publications, we have systematically summarized the method used for evaluating motor function, including the tests’ principles, procedures, evaluation indexes, advantages, and disadvantages. We then propose an operational program for evaluating the sarcopenia phenotype, which will be of help to researchers wishing to choose evaluation method appropriate to their specific research purposes. Further innovative technology for assessing motor function that could be instructive in the evaluation of skeletal muscle function and diagnosis of sarcopenia is summarized.